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KMID : 0861020220370060019
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Korea Journal of Herbology
2022 Volume.37 No. 6 p.19 ~ p.28
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Anti-inflammatory Activity of Cynanchi Atrati Radix Et Rhizoma Water Extracts via Regulation of MAPK in LPS-induced Murine Macrophage Cell Line, RAW 264.7
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Lee Sang-Ho
Kil ki-Jung
Yoo Ji-Hyun
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Abstract
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Objectives : To develop natural ingredients that help prevent or treat anti-inflammatory-related diseases and use themas basic data, we investigated anti-inflammatory activity of Cynanchi Atrati Radix Et Rhizoma water extracts(CWE)
in lipopolysaccharide(LPS)-induced murine macrophage cell line, RAW 264.7 cells.
Methods : The cell viabilities were evaluated with RAW 264.7 cells. The production of nitric oxide(NO), prostaglandin
E2(PGE2), pro-inflammatory cytokines such tumor necrotic factor(TNF)-α and interleukin(IL)-6 were assessed in
LPS-induced RAW 264.7 cell treated with CWE. Furthermore, the protein expression of inducible nitric oxide synthase
(iNOS), cyclooxygenase-2(COX-2), and mitogen-activated protein kinase(MAPK) were assessed by western blotting.
Results : In RAW 264.7 cell, the cell viability by CWE treatment was more than 98.4% at a concentration of 100-400
μg/mL. At a concentration of 800 ug/ml of CWE, the cell viability was as low as 86%. At doses of 100, 200 and 400
μg/mL, CWE inhibited the production of NO, PGE2, TNF-α and IL-6 in a dose-dependent manner and also decreased
the expression of iNOS and COX-2 from LPS-induced RAW 264.7 cells. In addition, CWE significantly inhibited the
MAPK pathway including decreased the phosphorylation of the p38, c-Jun N-terminal kinase(JNK) and extracellular
signal-regulated kinase(ERK1/2).
Conclusions : Our study provides evidence that CWE inhibits the production of main pro-inflammatory molecules in
LPS-induced RAW 264.7 cells via expression of p38, JNK, and ERK1/2 MAPK signaling pathways. Therefore, CWE
is expected to be widely used as a natural ingredient for anti-inflammatory functional foods or pharmaceuticals in
the future. 1)
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KEYWORD
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Cynanchi Atrati Radix Et Rhizoma, Anti-inflammatory, MAPK, RAW 264.7, lipopolysaccharide(LPS)
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